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Recognition of nonstructural protein-peptides by cytotoxic t-lymphocytes raised against japanese encephalitis-virus

Muralikrishna, K and Ramireddy, B and Ravi, V and Manjunath, R (1995) Recognition of nonstructural protein-peptides by cytotoxic t-lymphocytes raised against japanese encephalitis-virus. In: Microbiology and immunology, 39 (12). pp. 1021-1024.

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Abstract

We have previously reported that Lyt(2+) cytotoxic T lymphocytes (CTL) can be raised against Japanese encephalitis virus (JEV) in BALB/c mice. In order to confirm the presence of H-2K(d)-restricted CTL and to examine their cross-recognition of West Wile virus (WNV), we tested the capacity of anti-JEV CTL to lyse uninfected syngeneic target cells that were pulsed with synthetic peptides. The sequence of the synthetic peptides was predicted based upon the H-2K(d) binding consensus motif. We show here that preincubation of uninfected syngeneic targets (P388D1) with JEV NS1- and NS3-derived peptides [NS1 (891-899) and NS3 (1804-1812)], but not with JEV NS5-derived peptide [NS5 (3370-3378)], partially sensitized them for lysis by polyclonal anti-JEV CTL. These results indicate the CTL recognition of NS1- and NS3-derived peptides of JEV.

Item Type: Journal Article
Additional Information: copyright of this article belongs to Center for Academic Publications.
Keywords: Recombinant vaccinia viruses;protective immunity;nucleotide-sequence;mice;expression;induction;cells.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 18 Jan 2010 07:55
Last Modified: 18 Jan 2010 07:55
URI: http://eprints.iisc.ernet.in/id/eprint/25089

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