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Azospirillum lipoferum tRNAs: Fractionation and identification of tRNA species and the nucleotide sequence of tRNA(Asn) (QUU)

Sivakamasundari, R and Jayabaskaran, C (1996) Azospirillum lipoferum tRNAs: Fractionation and identification of tRNA species and the nucleotide sequence of tRNA(Asn) (QUU). In: Biochemistry & Molecular Biology International, 40 (6). 1221 -1226.

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Official URL: http://www.informaworld.com/smpp/750259880-1082822...

Abstract

Transfer RNAs of Azospirillum lipoferum were separated by two- dimensional gel electrophoresis and identified by aminoacylation. Thirty-six tRNA spots were resolved by this technique and twenty-six tRNA species have been identified. There are five tRNAs for Leu, four for Val, three for Pro, two each for Arg, Ile, Lys and Tyr, and one each for Ala, Asp, His, Phe, Ser and Thr. The tRNA(Asn) (QUU) was purified and its nucleotide sequence was determined. The A. lipoferum tRNA(Asn) (QUU) is 92% similar to B. subtilis tRNA(Asn) gene and two hypermodified nucleosides, queuosine (Q) and N-(9-beta-D Ribofuranosylpurine-6-YL) carbamoyl)-threonine (t(6)A) are present in this tRNA.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Taylore and Francis.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Feb 2010 08:19
Last Modified: 24 Feb 2010 08:19
URI: http://eprints.iisc.ernet.in/id/eprint/25607

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