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Immunospecific binding of tRNA precursors containing N6-(delta 2-isopentenyl) adenosine

Jayabaskaran, C (1984) Immunospecific binding of tRNA precursors containing N6-(delta 2-isopentenyl) adenosine. In: Biochemistry International, 8 (2). pp. 257-64.

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/6566571

Abstract

Antibodies specific for N6-(delta 2-isopentenyl) adenosine (i6A) were immobilized on Sepharose and this adsorbent (Sepharose-anti-i6A) was used to selectively isolate bacteriophage T4 tRNA precursors containing i6A/ms2i6A from an unfractionated population of 32P-labeled T4 RNAs. The results showed that antibodies to i6A selectively bound only those tRNA precursors containing i6A/ms2i6A. Binding of tRNA precursors by antibody and specificity of the binding was assessed by membrane binding using 32P-labeled tRNA precursor. Binding was highly specific for i6A/ms2i6A residues in the tRNA precursors. This binding can be used to separate modified from unmodified precursor RNAs and to study the biosynthetic pathways of tRNA precursors.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National Center for Biotechnology Information.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Feb 2010 08:10
Last Modified: 24 Feb 2010 08:10
URI: http://eprints.iisc.ernet.in/id/eprint/25612

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