Jayabaskaran, C (1984) Immunospecific binding of tRNA precursors containing N6-(delta 2-isopentenyl) adenosine. In: Biochemistry International, 8 (2). pp. 257-64.Full text not available from this repository.
Antibodies specific for N6-(delta 2-isopentenyl) adenosine (i6A) were immobilized on Sepharose and this adsorbent (Sepharose-anti-i6A) was used to selectively isolate bacteriophage T4 tRNA precursors containing i6A/ms2i6A from an unfractionated population of 32P-labeled T4 RNAs. The results showed that antibodies to i6A selectively bound only those tRNA precursors containing i6A/ms2i6A. Binding of tRNA precursors by antibody and specificity of the binding was assessed by membrane binding using 32P-labeled tRNA precursor. Binding was highly specific for i6A/ms2i6A residues in the tRNA precursors. This binding can be used to separate modified from unmodified precursor RNAs and to study the biosynthetic pathways of tRNA precursors.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to National Center for Biotechnology Information.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||24 Feb 2010 08:10|
|Last Modified:||24 Feb 2010 08:10|
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