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Biotransformation of cholesterol to diosgenin by freely suspended and immobilised cells of Dioscorea bulbifera L.

Jyothishwaran, G and Seetharam, YN (2008) Biotransformation of cholesterol to diosgenin by freely suspended and immobilised cells of Dioscorea bulbifera L. In: Journal of Asian Natural Products Research, 10 (2). pp. 139-145.

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Abstract

The cell suspension cultures, established from the friable callus which was risen from the nodal segments of Dioscorea bulbifera L. in Murashige-Skoog (MS) medium supplemented with indole-3-butryic acid (20 mg L- 1), was examined for cell growth in MS medium fed with cholesterol (50 mg L- 1 and 100 mg L- 1) after 8, 10, 12, 14, 16, and 18 days of culture. The growth index of the cell suspension culture on the 8th day was 1.2 and gradually inclined to 1.9 on the 16th day and remained the same at the 18th day. There is no marked difference in the cell growth of cholesterol-treated and control cell suspension culture. The maximum accumulation of diosgenin was noticed on the 14th day in control and cholesterol-treated cell suspension culture and immobilised cell cultures. The highest concentration of diosgenin, 2.94% and 2.14% dry weight, was obtained in immobilised cell culture and cell suspension culture treated with 100 mg L- 1 cholesterol, respectively.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Taylor and Francis Group.
Keywords: Biotransformation;Cholesterol;Dioscorea bulbifera;Diosgenin;Growth index;Immobilisation.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 08 Mar 2010 11:49
Last Modified: 08 Mar 2010 11:49
URI: http://eprints.iisc.ernet.in/id/eprint/26038

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