Das, Gautam and Thotala, Dinesh Kumar and Kapoor, Suman and Karunanithi, Sheelarani and Thakur, Suman S and Singh, N Sadananda and Varshney, Umesh (2008) Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli. In: EMBO Journal, 27 (6). pp. 840-851.Full text not available from this repository.
Translation initiation from the ribosomal P-site is the specialty of the initiator tRNAs (tRNA(fMet)). Presence of the three consecutive G-C base pairs (G29-C41, G30-C40 and G31-C39) in their anticodon stems, a highly conserved feature of the initiator tRNAs across the three kingdoms of life, has been implicated in their preferential binding to the P-site. How this feature is exploited by ribosomes has remained unclear. Using a genetic screen, we have isolated an Escherichia coli strain, carrying a G122D mutation in folD, which allows initiation with the tRNA(fMet) containing mutations in one, two or all the three G-C base pairs. The strain shows a severe deficiency of methionine and S-adenosylmethionine, and lacks nucleoside methylations in rRNA. Targeted mutations in the methyltransferase genes have revealed a connection between the rRNA modifications and the fundamental process of the initiator tRNA selection by the ribosome.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Nature Publishing Group.|
|Keywords:||S-adenosyl-L-methionine;5,10 methylene tetrahydrofolate dehydrogenase;methyltransferase;modified nucleoside;folD.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||30 Mar 2010 09:05|
|Last Modified:||30 Mar 2010 09:05|
Actions (login required)