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NSs Encoded by Groundnut Bud Necrosis Virus Is a ifunctional Enzyme

Lokesh, Bhushan and Rashmi, Panigrahi R and Amruta, Bhat S and Srisathiyanarayanan, Dharmaiah and Murthy, Mathur RN and Savithri, Handanahal S (2010) NSs Encoded by Groundnut Bud Necrosis Virus Is a ifunctional Enzyme. In: PLos One, 5 (3).

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Official URL: http://www.plosone.org/article/info%3Adoi%2F10.137...

Abstract

Groundnut bud necrosis virus (GBNV), a member of genus Tospovirus in the family Bunyaviridae, infects a large number of leguminosae and solanaceae plants in India. With a view to elucidate the function of nonstructural protein, NSs encoded by the small RNA genome (S RNA), the NSs protein of GBNV-tomato (Karnataka) [1] was over-expressed in E.coli and purified by Ni-NTA chromatography. The purified rNSs protein exhibited an RNA stimulated NTPase activity. Further, this activity was metal ion dependent and was inhibited by adenosine 5' (beta, gamma imido) triphosphate, an ATP analog. The rNSs could also hydrolyze dATP.Interestingly, in addition to the NTPase and dATPase activities, the rNSs exhibited ATP independent 5' RNA/DNA phosphatase activity that was completely inhibited by AMP. The 5' alpha phosphate could be removed from ssDNA, ssRNA, dsDNA and dsRNA thus confirming that rNSs has a novel 5' alpha phosphatase activity. K189A mutation in the Walker motif A (GxxxxGKT) resulted in complete loss of ATPase activity, but the 5'phosphatase activity was unaffected. On the other hand, D159A mutation in the Walker motif B (DExx) resulted in partial loss of both the activities. These results demonstrate for the first time that NSs is a bifunctional enzyme, which could participate in viral movement, replication or in suppression of the host defense mechanism.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Public Library of Science.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Biochemistry
Date Deposited: 08 Jun 2010 04:59
Last Modified: 08 Jun 2010 04:59
URI: http://eprints.iisc.ernet.in/id/eprint/27244

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