McMillan, DJ and Vu, T and Bramhachari, PV and Kaul, SY and Bouvet, A and Shaila, MS and Karmarkar, MG and Sriprakash, KS (2010) Molecular markers for discriminating Streptococcus pyogenes and S.dysgalactiae subspecies equisimilis. In: European Journal of Clinical Microbiology & Infectious Diseases, 29 (5). pp. 585-589.
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Given the increasing aetiological importance of Streptococcus dysgalactiae subspecies equisimilis in diseases which are primarily attributed to S. pyogenes, molecular markers are essential to distinguish these species and delineate their epidemiology more precisely. Many clinical microbiology laboratories rely on agglutination reactivity and biochemical tests to distinguish them. These methods have limitations which are particularly exacerbated when isolates with mixed properties are encountered. In order to provide additional distinguishing parameters that could be used to unequivocally discriminate these two common pathogens, we assess here three molecular targets: the speB gene, intergenic region upstream of the scpG gene (IRSG) and virPCR. Of these, the former two respectively gave positive and negative results for S. pyogenes, and negative and positive results for S. dysgalactiae subsp. equisimilis. Thus,a concerted use of these nucleic acid-based methods is particularly helpful in epidemiological surveillance to accurately assess the relative contribution of these species to streptococcal infections and diseases.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Springer.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||03 May 2010 06:29|
|Last Modified:||19 Sep 2010 06:01|
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