Spectral and kinetic characterization of 7,8-diaminopelargonic acid synthase from Mycobacterium tuberculosis

Bhor, Vikrant M and Dev, Sagarika and Vasanthakumar, Ganga Ramu and Surolia, Avadhesha (2006) Spectral and kinetic characterization of 7,8-diaminopelargonic acid synthase from Mycobacterium tuberculosis. In: IUBMB Life, 58 (4). pp. 225-233.

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Abstract

The indispensability of biotin for crucial processes like lipid biosynthesis coupled to the absence of the biotin biosynthesis pathway in humans make the enzymes of this pathway, attractive targets for development of novel drugs against numerous pathogens including M. tuberculosis. We report the spectral and kinetic characterization of the Mycobacterium tuberculosis 7,8-Diamino-pelargonic acid (DAPA) synthase, the second enzyme of the biotin biosynthesis pathway. In contrast to the E. coli enzyme, no quinonoid intermediate was detected during the steady state reaction between the enzyme and S-adenosyl-L-methionine (SAM). The second order rate constant for this half of the reaction was determined to be 1.75 +/- 0.11 M-1 s(-1). The K-m values for 7-keto-8-aminopelargonic acid (KAPA) and SAM are 2.83 mu M and 308.28 mu M, respectively whereas the V-max and k(cat) values for the enzyme are 0.02074 mu moles/min/ml and 0.003 s(-1), respectively. Our initial studies pave the way for further detailed mechanistic and kinetic characterization of the enzyme.

Item Type:	Journal Article
Additional Information:	Copy right of this article belongs to John Wiley and Sons.
Keywords:	Mycobacterium tuberculosis; biotin biosynthesis; 7-keto-8-aminopelargonic acid; 7,8-diaminopelargonic acid synthase; S-adenosyl-L-methionine; transimination; kinetics
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	22 Jun 2010 08:53
Last Modified:	19 Sep 2010 06:01
URI:	http://eprints.iisc.ernet.in/id/eprint/27474

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• Spectral and kinetic characterization of 7,8-diaminopelargonic acid synthase from Mycobacterium tuberculosis. (deposited 19 May 2010 05:14)
  • Spectral and kinetic characterization of 7,8-diaminopelargonic acid synthase from Mycobacterium tuberculosis. (deposited 22 Jun 2010 08:53) [Currently Displayed]

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