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Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes

Satish, PR and Surolia, Avadhesha (2002) Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes. In: Methods in Molecular Biology, 199 . pp. 193-202.

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Official URL: http://www.springerlink.com/content/uk11772r5835g3...

Abstract

The carbohydrate residues of glycosphingolipids were implicated in many biologic processes such as cell-to-cell interactions; and as receptors for some viruses, bacterial and plant toxins, hormones, and so forth, and invariably for all the lectins (1). However, their receptor functions remained poorly defined for a long time as they form micelles even at very low concentrations in aqueous medium. In micelles, the oligosaccharide chains are not expected to have a well defined orientation suitable for recognition by macromolecular ligands. This problem was overcome by incorporating them in model membranes, namely, the liposomes. The demonstration of lectin-glycolipid interaction using liposomal model membranes was a crucial development that established glycolipids as biological receptors. Moreover, glycolipid-bearing liposomes provide a convenient system for investigating the role of glycolipid density, orientation, and exposure of their oligosaccharide chains at the membrane interface relevant to their receptor function (2–4).

Item Type: Journal Article
Additional Information: Copyright of this article belongs to springer.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 19 May 2010 04:36
Last Modified: 19 May 2010 04:36
URI: http://eprints.iisc.ernet.in/id/eprint/27481

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