Satish, PR and Surolia, Avadhesha (2002) Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes. In: Methods in Molecular Biology, 199 . pp. 193-202.Full text not available from this repository. (Request a copy)
The carbohydrate residues of glycosphingolipids were implicated in many biologic processes such as cell-to-cell interactions; and as receptors for some viruses, bacterial and plant toxins, hormones, and so forth, and invariably for all the lectins (1). However, their receptor functions remained poorly defined for a long time as they form micelles even at very low concentrations in aqueous medium. In micelles, the oligosaccharide chains are not expected to have a well defined orientation suitable for recognition by macromolecular ligands. This problem was overcome by incorporating them in model membranes, namely, the liposomes. The demonstration of lectin-glycolipid interaction using liposomal model membranes was a crucial development that established glycolipids as biological receptors. Moreover, glycolipid-bearing liposomes provide a convenient system for investigating the role of glycolipid density, orientation, and exposure of their oligosaccharide chains at the membrane interface relevant to their receptor function (2–4).
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to springer.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||19 May 2010 04:36|
|Last Modified:||19 May 2010 04:36|
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