Pal, Debjani and Venkataraman, V and Mohan, Naga K and Chandra, Sharat H and Natarajan, Vasant (2004) A power-efficient thermocycler based on induction heating for DNA amplification by polymerase chain reaction. In: Review of Scientific Instruments, 75 (9). pp. 2880-2883.
We have built a thermocycler based on the principles of induction heating for polymerase chain reaction (PCR) of target sequences in DNA samples of interest. The cycler has an average heating rate of ~0.8 Ã�Â°C/s and a cooling rate of ~0.5 Ã�Â°C/s, and typically takes ~4 h to complete a 40-cycle PCR protocol. It is power-efficient (~6 W per reaction tube), micro-processor controlled, and can be adapted for battery operation. Using this instrument, we have successfully amplified a 350 bp segment from a plasmid and SRY, the human sex determining gene, which occurs as a single-copy sequence in genomic DNA of human males. The PCR products from this thermocycler are comparable to those obtained by the use of commercially available machines. Its easy front-end operation, low-power design, portability and low cost makes it suitable for diagnostic field applications of PCR.
|Item Type:||Journal Article|
|Additional Information:||Copyright for this article belongs to American Institute of Physics (AIP).|
|Department/Centre:||Division of Physical & Mathematical Sciences > Physics|
|Date Deposited:||07 Feb 2005|
|Last Modified:||19 Sep 2010 04:18|
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