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A simple and rapid method for quantification of hapten specific antibodies

Ghosh, Mridul and Surolia, A and Bachhawat, BK (1983) A simple and rapid method for quantification of hapten specific antibodies. In: Journal of Immunological Methods, 57 (1-3). pp. 295-300.

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Official URL: http://dx.doi.org/10.1016/0022-1759(83)90090-X

Abstract

A sensitive and simple method for quantification of antibodies against small molecules is described using DNP-lysozyme as the enzyme conjugate. The anti-DNP antiserum was raised against DNP-bovin serum albumin conjugate. Anti-DNP antibody or its monovalent fragment (Fab) reduced the enzyme activity of DNP-lysozyme conjugate in a concentration-dependent manner. The inhibition of enzyme activity is a specific measure of the antibody and Fab content of the sample. The specificity of the reaction was assessed by reduction of antibody-induced inhibition by DNP-lysine. The ability of DNP-lysine to reduce the antibody-induced inhibition of DNP-lysozyme activity also makes possible a sensitive assay for DNP-lysine.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Anti-hapten assay; DNP-lysine; anti-DNP antibody assay.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 18 May 2010 09:55
Last Modified: 18 May 2010 09:55
URI: http://eprints.iisc.ernet.in/id/eprint/27548

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