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The specificity of the colour reaction between ammonia and 3-phenyl-2-thiohydantoin

Reddy, K Nagendra Nath and Ramchandrsan, LK (1965) The specificity of the colour reaction between ammonia and 3-phenyl-2-thiohydantoin. In: Analytica Chimica Acta, 32 . pp. 435-447.

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Official URL: http://dx.doi.org/10.1016/S0003-2670(00)88967-9

Abstract

The colour reaction between 3-phenyl-2-thiohydantoin and ammonia is studied quantitatively. Determinations of 0.1–0.6 μmoles of 3-phenyl-2-thiohydantoin are possible with a precision close to 2%. In analyses of amino acid mixtures for glycine after conversion to 3-phenyl-2-thiohydantoin, only derivatives of serine and threonine interfere to a slight extent. The specificity of the primary colour reaction with ammonia, and the structural requirements for it are discussed; a structure for the pigment species is proposed.

Item Type: Journal Article
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Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 19 May 2010 06:06
Last Modified: 19 Sep 2010 06:07
URI: http://eprints.iisc.ernet.in/id/eprint/27920

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