ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

An indole oxidase isolated from the leaves of Tecoma stans

Nair, Madhusudanan P and Vaidyanathan, CS (1964) An indole oxidase isolated from the leaves of Tecoma stans. In: Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 81 (3). pp. 496-506.

[img] PDF
An.pdf - Published Version
Restricted to Registered users only

Download (528Kb) | Request a copy
Official URL: http://dx.doi.org/10.1016/0926-6569(64)90134-8

Abstract

The presence of an indole oxidase (indole: O2 oxidoreductase) was detected in the leaf extracts of Tecoma stans. The end product of the reaction was identified as anthranil. Formylaminobenzaldehyde, and o- aminobenzaldehyde were detected as intermediates in the overall conversion. Oxygen-uptake studies established that 3 atoms of oxygen were consumed in the formation of anthranil form I molecule of indole. The enzyme showed an absolute requirement for FAD and Cu2+ for maximum activity. FMN was ineffective as a cofactor. The enzyme had an optimum pH of 5.0. Inhibition studies with GSH and p-chloromericuribenzoate showed that a sulfhydrylcupric-ion complex at the active centre is highly essential.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 May 2010 07:27
Last Modified: 19 Sep 2010 06:07
URI: http://eprints.iisc.ernet.in/id/eprint/28083

Actions (login required)

View Item View Item