Fatty acid component of vitamin A ester in sheep liver

Rao, KS and Seshadri Sastry, P (1961) Fatty acid component of vitamin A ester in sheep liver. In: Archives of Biochemistry and Biophysics, 95 (2). pp. 285-289.

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Abstract

Vitamin A, when extracted along with other lipids from sheep liver, had an E1cm.1% value of 14.4, which was raised to 45.57 on removal of the phospholipids by cold acetone. Selective hydrolysis of triglycerides by an extract of acetone-dried sheep pancreas in the presence of HgCl2 as inhibitor of vitamin A esterase, followed by chromatography through alumina gave a product with E1cm.1% value of 276. This on chromatography through magnesium oxide raised the E1cm.1, value to 601.5, representing 64% pure vitamin A ester calculated as palmitate, and the total recovery was 23% of the starting oil. The purified ester preparation, when subjected to reverse-phase chromatography on silicone-impregnated paper, gave a single ultraviolet fluorescent band. The fluorescent band on hydrolysis gave only one fatty acid. This was conclusively identified to be palmitic acid.

Item Type:	Journal Article
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	16 Jun 2010 07:35
Last Modified:	19 Sep 2010 06:09
URI:	http://eprints.iisc.ernet.in/id/eprint/28451

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