Mondal, PP and Mandal, S and Diaspro, A (2010) Note: Dynamic point spread function for single and multiphoton fluorescence microscopy. In: Review of Scientific Instruments, 81 (4).
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We propose and demonstrate a dynamic point spread function (PSF) for single and multiphoton fluorescence microscopy. The goal is to generate a PSF whose shape and size can be maneuvered from highly localized to elongated one, thereby allowing shallow-to-depth excitation capability during active imaging. The PSF is obtained by utilizing specially designed spatial filter and dynamically altering the filter parameters. We predict potential applications in nanobioimaging and fluorescence microscopy.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to American Institute of Physics.|
|Department/Centre:||Division of Physical & Mathematical Sciences > Instrumentation and Applied Physics (Formally ISU)|
|Date Deposited:||13 Jul 2010 07:20|
|Last Modified:||19 Sep 2010 06:10|
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