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A novel conotoxin modulating sodium channels

Balaram, Padmanabhan and Krishnan, Kozhalmannom Subramaniasastry (2005) A novel conotoxin modulating sodium channels. Patent Number(s) WO 2005030801 A1. Patent Assignee(s) National Centre for Biological Sciences.

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Abstract

A 26 residue peptide (Am2766) with the sequence CKQAGESCDIFSQNCCVGTCAFICE-NH2 has been isolated and purified from the venom of the molluscivorous snail, Conus amadis, collected of the southeastern coast of India. Chemical modification and mass spectrometric studies establish that Am2766 has three disulfide bridges. Cterminal amidation has been demonstrated by mass measurements on the C-terminal fragments obtained by proteolysis. Sequence alignments establish that Am2766 belongs to the 6-conotoxin family. Am2766 inhibits the decay of the sodium current in brain rNavl .2a voltage-gated Na+ channel, stably expressed in Chinese hamster ovary (CHO) cells. Unlike 6-conotoxins have previously been isolated from molluscivorous snails, Am 2766 inhibits inactivation of mammalian sodium channel.

Item Type: Patent
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 15 Apr 2005
Last Modified: 19 Sep 2010 04:18
URI: http://eprints.iisc.ernet.in/id/eprint/2945

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