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Studies on non-specific interference due to serum in the avidin-biotin microELISA for monkey chorionic gonadotropin and a method for its elimination

Jagannadha, Rao A and Chakraborti, R (1989) Studies on non-specific interference due to serum in the avidin-biotin microELISA for monkey chorionic gonadotropin and a method for its elimination. In: Journal of Immunological Methods, 125 (1-2). pp. 261-264.

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Official URL: http://dx.doi.org/10.1016/0022-1759(89)90101-4

Abstract

A study has been carried out on the non-specific interference due to serum in the avidin biotin micro-ELISA for monkey chorionic gonadotropin. Results suggest that it is not due to any proteolytic activity in the serum, but immunoglobulin or associated factors interfering at the level of antigen-antibody interaction. This interference was eliminated by heating samples at 60°C for 30 min.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Elisa;γ-Globulin,bovine;Gonadotrophin.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 16 Jul 2010 06:19
Last Modified: 16 Jul 2010 06:19
URI: http://eprints.iisc.ernet.in/id/eprint/30317

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