Rajasekharan, Ram and Kanchan, Bhardwaj (2002) A Process of isolation and utiliztion of Rice Bran Lipase. Patent Number(s) WO 02101033 A1. Patent Assignee(s) Indian Institute of Science;CB Bio Products India Pvt. Ltd.
Disclosed herein is a novel isolated from nomocotyledon plant sources such as rice bran having the following properties: a) isolated enzyme is a glycoprotein; b) isolated enzyme is stable and catalytically active at high temperatures; c) isolated enzyme is stable and catalytically active at alkaline pH; d) isolated enzyme has molecular weight range of 9 to 12-kDa; e) isolated enzyme is inhibited by inhibitors of serine proteases such as Diisopropylfluorophosphate; f) Isolated enzyme that can utilize both triacylglycerol and phospholipids. This invention also relates to a process of purifying the lipase described above using the following steps: a) delipidation of the plant extract using organic solvents; b) Filtration of the delipidated extract on a suitable matrix; c) Purification of the enzyme using hydrophobic column matrices; d) characterization of the purified product by known methods.
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||11 Apr 2005|
|Last Modified:||19 Sep 2010 04:18|
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