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Quantitation of serological cross-reactivity between two geographical isolates of Oryctes baculovirus by a modified ELISA

Mohan, KS and Gopinathan, KP (1989) Quantitation of serological cross-reactivity between two geographical isolates of Oryctes baculovirus by a modified ELISA. In: Journal of Virological Methods, 24 (1-2). pp. 203-213.

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Official URL: http://dx.doi.org/10.1016/0166-0934(89)90022-0

Abstract

An assay was developed for quantitation of the antigenic relationship between viruses, by modification of the indirect ELISA. The principle of this method is to estimate the epitopes not shared between the related viruses, after titration of the antibodies specific to the common epitopes as in a blocking ELISA. In practice, varying concentrations of purified virus are preincubated with a fixed dilution of heterologous or homologous antiserum and the unbound antibodies present in the mixture are back titrated with virus particles bound to microtitre plates. The antigenic relationship is described in terms of differentiation index (DI) and total antigenic reactivity (TAR). This method has been used to quantitate cross-reactivity between two geographically different isolates of Oryctes baculovirus.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Modified ELISA;Antigenic cross-reactivity;Oryctes baculovirus isolate;Total antigenic reactivity.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Others
Date Deposited: 24 Aug 2010 07:11
Last Modified: 24 Aug 2010 07:11
URI: http://eprints.iisc.ernet.in/id/eprint/31390

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