Nagpal, S and Shanthi, KN and Kori, R and Schroder, H and Metcalfe, DD and Rao, Subba PV (1989) Induction of allergen-specific IgE and IgG responses by anti-idiotypic antibodies. In: The Journal of Immunology, 142 (10). pp. 3411-3415.
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In order to explore idiotypic, anti-idiotypic, and anti-anti-idiotypic responses to allergens, BALB/c mice were immunized with affinity- purified human idiotypic antibodies directed against a highly purified shrimp allergen. This resulted in the production of anti-idiotypic antibodies which were quantitated by using rabbit idiotypic antibodies raised against the same purified allergen. The mouse anti-idiotypic antibodies recognized shrimp-specific human idiotypic antibodies of the IgE isotype from 18 of 20 individuals, and IgG antibodies from 14 of 20 shrimp-sensitive patients. Immunization of BALB/c mice with affinity- purified, allergen-specific anti-idiotypic antibodies induced anti- allergen IgE and IgG responses in the absence of the allergen. This paper thus presents evidence that anti-idiotypic antibodies raised against allergen-specific idiotypic antibodies may substitute for the original allergen in the induction of allergen-specific idiotypic antibodies. The demonstration of shared idiotopes on IgG and IgE antibodies in the sera of shrimp-sensitive patients supports the use of allergen-specific anti-idiotypic antibodies as surrogate allergens.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to American Association of Immunologists.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||19 Aug 2010 12:56|
|Last Modified:||19 Sep 2010 06:14|
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