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Sorghum proteinase inhibitors: purification and some biochemical properties

Kumar, Harish PM and Virupaksha, Tumkur K and Vithayathil, Paul Joseph (1978) Sorghum proteinase inhibitors: purification and some biochemical properties. In: International Journal of Peptide & Protein Research, 12 (4). pp. 185-196.

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1399-...

Abstract

An investigation has been carried out on the proteinase inhibitors of grain sorghum (Sorghum bicolor (L.) Moench). One of the inhibitors has been isolated in a pure form and characterized. The proteinase inhibitor was extracted from the acetone-defatted sorghum meal and purified by selective thermal denaturation, ammonium sulfate fractionation, Sephadex gel filtration and DEAE-cellulose chromatography (DEAE-preparation II). This preparation was demonstrated to be a mixture of three inhibitor components by polyacrylamide disc gel electrophoresis. Further resolution of this mixture into Inhibitors I to III was achieved by QAE-Sephadex chromatography. Sorghum Inhibitor III was homogeneous by the criteria of disc gel electrophoresis and has been more fully characterized. A molecular weight of 25,000 was obtained for Inhibitor III by gel filtration and was in agreement with the value calculated from the amino acid composition of the inhibitor. The N-terminal amino acid residue of Inhibitor III, a single chain protein, was isoleucine. Sorghum proteinase inhibitors inhibit specifically the serine proteinases and are inactive towards the other classes of proteinases. Inhibitor III is primarily a chymotrypsin inhibitor, whereas Inhibitors I and II inhibit both trypsin and chymotrypsin.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Munksgaard int publ ltd.
Keywords: Isolation of sorghum isoinhibitors;plant Proteinasenhibitors;proteinase inhibitors;sorghum bicolor (L) moench;sorghum proteinase inhibitors;specificity of sorghum inhibitors.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 23 Aug 2010 05:32
Last Modified: 23 Aug 2010 05:32
URI: http://eprints.iisc.ernet.in/id/eprint/31471

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