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Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones

Diallo, Adama and Barrett, Thomas and Barbron, Monique and Subbarao, Shaila M and Taylor, William P (1989) Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones. In: Journal of Virological Methods, 23 (2). pp. 127-136.

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Official URL: http://dx.doi.org/10.1016/0166-0934(89)90126-2

Abstract

The morbilliviruses which infect ruminants, rinderpest (RPV) and peste des petits ruminants (PPRV), are difficult to distinguish serologically. They can be distinguished by differential neutralisation tests and by the migration of the major virus structural protein, the nucleocapsid protein, on polyacrylamide gels. Both these methods are time consuming and require the isolation of live virus for identification; they are not suitable for analysis of material directly from post-mortem specimens. We describe a rapid method for differential diagnosis of infections caused by RPV or PPRV, which uses specific cDNA probes, derived from the mRNAs for the nucleocapsid protein of each virus, which can be used to distinguish unequivocally the two virus types rapidly.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier science.
Keywords: Morbillivirus;Rinderpest;PPR;Nucleocapsid gene;Hybridization.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 26 Aug 2010 06:39
Last Modified: 26 Aug 2010 06:39
URI: http://eprints.iisc.ernet.in/id/eprint/31538

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