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Interaction of 3-hydroxybenzoate-6-hydroxylase with cibacron blue

Sumathi, S and Dasgupta, Dipak (2006) Interaction of 3-hydroxybenzoate-6-hydroxylase with cibacron blue. In: Journal of Enzyme Inhibition and Medicinal Chemistry, 21 (1). pp. 47-53.

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Official URL: http://informahealthcare.com/doi/abs/10.1080/02781...

Abstract

Cibacron blue is a potent inhibitor of 3-HBA-6-hydroxylase at a concentration < 1 mu M. Kinetic analyses revealed that at a concentration below 0.5 mu M the dye behaves as an uncompetitive inhibitor with respect to 3-HBA and competes with NADH for the same site on the enzyme. The alteration of the near-UV CD spectrum and quenching of the emission fluorescence of the enzyme by cibacron blue indicates a significant alteration in the environment of aromatic amino acid residues due to a stacking interaction and subtle conformatiodnal changes in the enzyme. The concentration-dependent quenching of the intrinsic fluorescence of the enzyme by cibacron blue was employed to determine the binding parameters such as association constant (K-a) and stoichiometry (r) for the enzyme-dye complex.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Taylore and Francies.
Keywords: cibacron blue; circular dichroism; competitive; flavin; fluorescence; 3-hydroxybenzoate; inhibition; uncompetitive; monooxygenase; 3-hydroxybenzoate-6-hydroxylase.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 17 Sep 2010 04:59
Last Modified: 17 Sep 2010 04:59
URI: http://eprints.iisc.ernet.in/id/eprint/31729

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