# Binding Constant Measurement by Hyper-Rayleigh Scattering: Bilirubin-Human Serum Albumin Binding as a Case Study

Ranjini, Arumugam Sri and Das, Puspendu K and Balaram, Padmanabhan (2005) Binding Constant Measurement by Hyper-Rayleigh Scattering: Bilirubin-Human Serum Albumin Binding as a Case Study. In: Journal of Physical Chemistry B, 109 (12). pp. 5950-5953.

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## Abstract

In this paper, a new application of the hyper-Rayleigh scattering technique in determining multiple binding constants of a small molecule like bilirubin to a macromolecule like the protein human serum albumin has been demonstrated. Human serum albumin has two binding sites for bilirubin, and the binding constants have been measured by carrying out a second harmonic titration of the protein against bilirubin and vice versa. The measured binding constants $K_1 = 1.5 \pm 0.43 \times 10^7 M^-^1$ and $K_2 = 1.01 \pm 0.16 \times 10^6 M^-^1$ agree well with the reported values obtained by other methods.

Item Type: Journal Article The copyright for this article belongs to American Chemical Society. Division of Biological Sciences > Molecular Biophysics UnitDivision of Chemical Sciences > Inorganic & Physical Chemistry 19 May 2005 19 Sep 2010 04:18 http://eprints.iisc.ernet.in/id/eprint/3217