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Occurrence of Lipid Peroxidation in Brain Microsomes in the Presence of NADH and Vanadate

Patole, MS and Ramasarma, T (1988) Occurrence of Lipid Peroxidation in Brain Microsomes in the Presence of NADH and Vanadate. In: Journal of Neurochemistry, 51 (2). pp. 491-496.

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1471-...

Abstract

Oxidation of NADH by rat brain microsomes was stimulated severalfold on addition of vanadate. During the reaction, vanadate was reduced, oxygen was consumed, and H2O2 was generated with a stoichiometry of 1:1 for NADH/O2, as in the case of other membranes. Extra oxygen was found to be consumed over that needed for H2O2 generation specifically when brain microsomes were used. This appears to be due to the peroxidation of lipids known to be accompanied by a large consumption of oxygen. Occurrence of lipid peroxidation in brain microsomes in the presence of NADH and vanadate has been demonstrated. This activity was obtained specifically with the polymeric form of vanadate and with NADH, and was inhibited by the divalent cations Cu2+, Mn2+, and Ca2+, by dihydroxy-phenolic compounds, and by hemin in a concentration-dependent fashion. In the presence of a small concentration of vanadate, addition of an increasing concentration of Fe2+ gave increasing lipid peroxidation. After undergoing lipid peroxidation in the presence of NADH and vanadate, the binding of quinuclidinyl benzylate, a muscarinic antagonist, to brain membranes was decreased.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to John Wiley and Sons.
Keywords: Lipid peroxidation;Brain microsomes;Vanadate;NADH.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Sep 2010 10:49
Last Modified: 24 Sep 2010 10:49
URI: http://eprints.iisc.ernet.in/id/eprint/32410

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