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Plasmid DNA mediated transfer of the herpes simplex virus thymidine kinase gene to a new bromodeoxyuridine resistant variant of human primary lung carcinoma cells

Manjunath, GS and Dufresne, MJ (1988) Plasmid DNA mediated transfer of the herpes simplex virus thymidine kinase gene to a new bromodeoxyuridine resistant variant of human primary lung carcinoma cells. In: Biochemistry International, 16 (1). pp. 149-156.

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Official URL: http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed...

Abstract

A human primary lung carcinoma cell line (HPL-R1) established from the tumor biopsy of a lung cancer patient, lacking in cytochrome P1-450 [aryl hydrocarbon (benzo[a]pyrene) hydroxylase (AHH)], was cloned and used to obtain variants deficient in the expression of thymidine-kinase via treatment with 5-bromo-2'-deoxyuridine, and selection for drug resistance phenotype. The variant cell line, precharacterized for thymidine kinase negative phenotype, was transfected with the thymidine kinase gene bearing p R-tk and px1-tk plasmids. Transfections from both the plasmids, demonstrated a frequency of 5.5 X 10(-5). The transfectants showed a 76-100% retention of the transferred phenotype. These data suggest that transfection in variant human cells can approach significant levels of stability observed with rodent cell recipients.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Press Aust.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 29 Sep 2010 10:09
Last Modified: 29 Sep 2010 10:09
URI: http://eprints.iisc.ernet.in/id/eprint/32499

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