Manjunath, GS and Dufresne, MJ (1988) Plasmid DNA mediated transfer of the herpes simplex virus thymidine kinase gene to a new bromodeoxyuridine resistant variant of human primary lung carcinoma cells. In: Biochemistry International, 16 (1). pp. 149-156.Full text not available from this repository.
A human primary lung carcinoma cell line (HPL-R1) established from the tumor biopsy of a lung cancer patient, lacking in cytochrome P1-450 [aryl hydrocarbon (benzo[a]pyrene) hydroxylase (AHH)], was cloned and used to obtain variants deficient in the expression of thymidine-kinase via treatment with 5-bromo-2'-deoxyuridine, and selection for drug resistance phenotype. The variant cell line, precharacterized for thymidine kinase negative phenotype, was transfected with the thymidine kinase gene bearing p R-tk and px1-tk plasmids. Transfections from both the plasmids, demonstrated a frequency of 5.5 X 10(-5). The transfectants showed a 76-100% retention of the transferred phenotype. These data suggest that transfection in variant human cells can approach significant levels of stability observed with rodent cell recipients.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Academic Press Aust.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||29 Sep 2010 10:09|
|Last Modified:||29 Sep 2010 10:09|
Actions (login required)