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Purification of goat serum retinol-binding protein and preparation of its antibodies

Sreekrishna, K and Cama, HR (1978) Purification of goat serum retinol-binding protein and preparation of its antibodies. In: Proceedings:plant sciences, 87 (7). pp. 205-212.

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Official URL: http://www.springerlink.com/content/06517jm6708437...

Abstract

The method for the purification of goat serum retinol-binding protein consists of DEAE-cellulose chromatography of the serum followed by preparative polyacrylamide disc gel electrophoresis. After electrophoresis, the retinol-binding protein containing zone is identified by the specific fluorescence of retinol. For raising the antibodies, the portion of the gel containing retinol binding protein is homogenized and injected intradermally and intramuscularly to rabbits. The availability of this simple method for the isolation of retinol-binding protein and production of its antibodies enables the development of a radioimmunoassay for this protein.

Item Type: Journal Article
Additional Information: Copyright of this article belong to Indian Academy of Sciences.
Keywords: Retinol -retinol-binding protein -prealbumin -antibodies.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 29 Sep 2010 11:22
Last Modified: 29 Sep 2010 11:22
URI: http://eprints.iisc.ernet.in/id/eprint/32522

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