Kumar, Madan A and Nayak, R (1988) Rapid isolation of lysyl-tRNA synthetase from rat liver. In: Biochemical and Biophysical Research Communications, 152 (2). 593 -597.
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The high molecular weight aminoacyl-tRNA synthetase complex (the 24S complex) was isolated from rat liver by ultracentrifugation. The lysyl-tRNA synthetase (E.C. 220.127.116.11) was selectively dissociated by hydrophobic interaction chromatography on 1,6 diaminohexyl agarose followed by hydroxylapatite chromatography and DEAE chromatography. The lysyl-tRNA synthetase dissociated from the 24S synthetase complex was purified approximately to 2700 fold with 14% yield.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier science.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||29 Sep 2010 09:38|
|Last Modified:||29 Sep 2010 09:38|
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