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Rapid isolation of lysyl-tRNA synthetase from rat liver

Kumar, Madan A and Nayak, R (1988) Rapid isolation of lysyl-tRNA synthetase from rat liver. In: Biochemical and Biophysical Research Communications, 152 (2). 593 -597.

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Official URL: http://dx.doi.org/10.1016/S0006-291X(88)80079-2

Abstract

The high molecular weight aminoacyl-tRNA synthetase complex (the 24S complex) was isolated from rat liver by ultracentrifugation. The lysyl-tRNA synthetase (E.C. 6.1.1.6) was selectively dissociated by hydrophobic interaction chromatography on 1,6 diaminohexyl agarose followed by hydroxylapatite chromatography and DEAE chromatography. The lysyl-tRNA synthetase dissociated from the 24S synthetase complex was purified approximately to 2700 fold with 14% yield.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier science.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 29 Sep 2010 09:38
Last Modified: 29 Sep 2010 09:38
URI: http://eprints.iisc.ernet.in/id/eprint/32526

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