ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Temperature and thiol-induced desensitization of a Ca2+-sensitive cyclic-3',5'-nucleotide phosphodiesterase from sheep lung

Radhakrishnan, TS and Savithri, HS and Rao, NA and Venkitasubramanian, TA (1988) Temperature and thiol-induced desensitization of a Ca2+-sensitive cyclic-3',5'-nucleotide phosphodiesterase from sheep lung. In: Biochemistry International, 17 (5). pp. 927-933.

Full text not available from this repository.
Official URL: http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed...

Abstract

Ca2+-sensitivity of sheep lung cyclic-3',5'-nucleotide phosphodiesterase is provided by endogenous tightly bound calmodulin. The calcium sensitivity of a highly purified enzyme was desensitized by increasing the assay temperature. It could also be desensitized to Ca2+-activation by thiols such as dithiothreitol. The thiol-induced desensitization could be partially reversed by dialysis and almost completely reversed by dilution. The results presented in this paper indicate that thiols are possibly involved in the interaction of calmodulin with cyclic-3',5'-nucleotide phosphodiesterase. This is the first report on temperature and thiol-induced desensitization of Ca2+-sensitivity of a cyclic-3',5'-nucleotide phosphodiesterase.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Press Aust.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 11 Oct 2010 08:51
Last Modified: 11 Oct 2010 08:51
URI: http://eprints.iisc.ernet.in/id/eprint/33014

Actions (login required)

View Item View Item