Karanth, Megha N and Sarma, Siddhartha P (2010) H-1, C-13, N-15 assignments of the dimeric regulatory subunit (ilvN) of the E. coli AHAS I. In: Biomolecular NMR Assignments, 4 (2). pp. 131-133.
subunit.pdf - Published Version
Restricted to Registered users only
Download (264Kb) | Request a copy
Acetohydroxyacid synthase (AHAS) is an enzyme involved in the biosynthesis of the branched chain amino acids viz, valine, leucine and isoleucine. The activity of this enzyme is regulated through feedback inhibition by the end products of the pathway. Here we report the backbone and side-chain assignments of ilvN, the 22 kDa dimeric regulatory subunit of E. coli AHAS isoenzyme I, in the valine bound form. Detailed analysis of the structure of ilvN and its interactions with the catalytic subunit of E. coli AHAS I will help in understanding the mechanism of activation and regulation of the branched chain amino acid biosynthesis.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Springer.|
|Keywords:||AHAS I; ilvN; NMR; E. coli; Valine; Branched chain amino acid biosynthesis.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||21 Oct 2010 05:14|
|Last Modified:||21 Oct 2010 05:14|
Actions (login required)