Kantharaj, GR and Mahadevan, S and Padmanaban, G (1979) Early biochemical events during adventitious root initiation in the hypocotyl of Phaseolus vulgaris. In: Phytochemistry, 18 (3). pp. 383-387.
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Indole butyric acid (IBA) initiates roots in the hypocotyl tissue of Phaseolus vulgaris (French bean). The response is dependent on the concentration of IBA and the duration of exposure to the hormone. IBA enhances the rate of total protein synthesis in ca 30 min after exposure of the hypocotyl segments to the hormone. There is no detectable change in total or poly(A)-containing RNA synthesis in this period although significant increases are seen 2 hr after hormone pre-treatment. The early IBA-mediated increase in protein synthesis (30 min) is not sensitive to Actinomycin D but the antibiotic blocks the increase manifested 2 hr after hormone pre-treatment. Inhibition of early protein synthesis by cycloheximide depresses and delays root initiation. Cytosol prepared from IBA-treated hypocotyl tissue stimulates protein synthesis in vitro to a greater extent than that of the control.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier Science.|
|Keywords:||Phaseolus vulgaris; Leguminosae; French bean; auxin; root initiation; protein synthesis: RNA synthesis.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||20 Oct 2010 06:52|
|Last Modified:||14 Nov 2011 05:31|
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