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Photobleaching reduced fluorescence correlation spectroscopy

Mondal, Partha P and Gilbert, Richard J and So, Peter TC (2010) Photobleaching reduced fluorescence correlation spectroscopy. In: Applied Physics Letters, 97 (10).

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Abstract

A dual beam excitation-depletion pulse technique is proposed for photobleaching reduced fluorescence correlation spectroscopy (FCS). Excitation pulse promote the molecules to the excited singlet state (S-1), a fraction of that population goes to energetically favorable metastable triplet state (T-1) due to strong intersystem crossing. The depletion pulse followed by excitation pulse instantaneously depletes the triplet states thereby recycling the bleached molecules back to the ground state (S-0). FCS study on diffusing Fluorescein and Rh6G molecules show more than 95% reduction in triplet state population and the associated photobleaching. (c) 2010 American Institute of Physics.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to American Institute of Physics.
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation and Applied Physics (Formally ISU)
Date Deposited: 02 Nov 2010 06:08
Last Modified: 02 Nov 2010 06:08
URI: http://eprints.iisc.ernet.in/id/eprint/33578

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