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Destruction Of Rat-Liver Microsomal Cytochrome-P-450 Invitro By A Monoterpene Ketone, Pulegone A Hepatotoxin

Moorthy, B and Madyastha, P and Madyastha, KM (1991) Destruction Of Rat-Liver Microsomal Cytochrome-P-450 Invitro By A Monoterpene Ketone, Pulegone A Hepatotoxin. In: Indian Journal of Chemistry - Section B: Organic and Medicinal Chemistry, 30 (2). pp. 138-146.

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Official URL: http://www.niscair.res.in/ScienceCommunication/Res...

Abstract

Significant destruction (68%) of liver microsomal cytochrome P-450 and homogeneous cytochrome P-450 purified from PB-treated rats is noticed upon incubation with 10 mM pulegone at 37-degrees-C for 30 min. There is also a concomitant loss of heme. The destructive phenomenon does not require metabolic activation of pulegone. The destruction of purified cytochrome P-450 is time-dependent and saturable. Structure-activity studies suggest that an alpha-isopropylidine ketone unit with a methyl positioned para to the isopropylidine group as in pulegone is necessary for the in vitro destruction of cytochrome P-450. SKF-525A at a concentration of 4-mM obliterates the destruction of cytochrome P-450 by pulegone. Experiments with C-14-pulegone suggest that pulegone or its rearranged product binds covalently to the prosthetic heme of cytochrome P-450.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Keywords: Metabolism;Enzymes;Invivo;Heme;Inactivation;Reductase.
Department/Centre: Division of Chemical Sciences > Organic Chemistry
Date Deposited: 10 Nov 2010 07:30
Last Modified: 10 Nov 2010 07:30
URI: http://eprints.iisc.ernet.in/id/eprint/33733

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