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Development of a probe-based blotting technique for the detection of Tobacco streak virus

Sarovar, B and Saigopal, DVR (2010) Development of a probe-based blotting technique for the detection of Tobacco streak virus. In: Acta Virologica, 54 (3). pp. 221-224.

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Abstract

Digoxigenin (DIG)-labeled DNA probe was developed for a sensitive and rapid detection of the Tobacco streak virus (TSV) isolates in India by dot-blot and tissue print hybridization techniques. DIG-labeled DNA probe complementary to the coat protein (CP) region of TSV sunflower isolate was designed and used to detect the TSV presence at field levels. Dot-blot hybridization was used to check a large number of TSV isolates with a single probe. In addition, a sensitivity of the technique was examined with the different sample extraction methods. Another technique, the tissue blot hybridization offered a simple, reliable procedure and did not require a sample processing. Thus, both non-radioactively labeled probe techniques could facilitate the sample screening during TSV outbreaks and offer an advantage in quarantine services.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Electronic Press.
Keywords: Tobacco streak virus isolates; DIG-labeled DNA probe; dot-blot hybridization; tissue print hybridization technique; diagnosis
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 19 Nov 2010 10:49
Last Modified: 19 Nov 2010 10:49
URI: http://eprints.iisc.ernet.in/id/eprint/33852

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