Murthy, Usha S and Sreekrishna, K and Adiga, PR (1979) A simplified method for the purification of riboflavin-binding protein from hen's egg yolk. In: Analytical Biochemistry, 92 (2). pp. 345-350.
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A simple and efficient procedure for the purification of the riboflavin-binding protein from hen's egg yolk is described. This method involves the removal by exclusion of lipoproteins and subsequent fractionation of soluble yolk proteins held on a DEAE-cellulose column by a salt gradient which is followed by purification by gel filtration on Sephadex G-100. The protein thus isolated is homogeneous by various physicoehemical, immunological, and functional criteria.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier Science.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||30 Nov 2010 12:16|
|Last Modified:||30 Nov 2010 12:16|
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