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The effects of beta-mercaptoethanol and sodium dodecyl sulfate on the Humicola insolens beta-glucosidase

Rao, US and Murthy, SK (1991) The effects of beta-mercaptoethanol and sodium dodecyl sulfate on the Humicola insolens beta-glucosidase. In: Biochemistry International, 23 (2). 343 -348.

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Official URL: http://journal.library.iisc.ernet.in/archives.html

Abstract

Hydrolysis of p-nitrophenyl-beta-D-glucoside by the beta-glucosidase of a thermophilic and cellulolytic fungus, Humicola insolens was stimulated by two-fold in the presence of high concentrations of beta-mercaptoethanol. This enzyme did not have any free sulfhydryl groups and high concentrations of beta-mercaptoethanol (5% v/v) reduced all of the three disulfide bonds present in the enzyme. In contrast, the hydrolysis of cellobiose and cellulose polymers was inhibited by 50% under the same conditions. Sodium dodecyl sulfate (1% w/v) even in combination with beta-mercaptoethanol did not show any significant effects on this enzyme. These unusual properties suggest that this enzyme may be of significant importance for understanding the structure of the enzyme.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Press Aust.
Keywords: Thermoascus-Aurantiacus;Thermophilic Fungus;Purification; Endocellulase.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 07 Dec 2010 09:43
Last Modified: 07 Dec 2010 09:43
URI: http://eprints.iisc.ernet.in/id/eprint/34236

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