Rao, US and Murthy, SK (1991) The effects of beta-mercaptoethanol and sodium dodecyl sulfate on the Humicola insolens beta-glucosidase. In: Biochemistry International, 23 (2). 343 -348.Full text not available from this repository.
Hydrolysis of p-nitrophenyl-beta-D-glucoside by the beta-glucosidase of a thermophilic and cellulolytic fungus, Humicola insolens was stimulated by two-fold in the presence of high concentrations of beta-mercaptoethanol. This enzyme did not have any free sulfhydryl groups and high concentrations of beta-mercaptoethanol (5% v/v) reduced all of the three disulfide bonds present in the enzyme. In contrast, the hydrolysis of cellobiose and cellulose polymers was inhibited by 50% under the same conditions. Sodium dodecyl sulfate (1% w/v) even in combination with beta-mercaptoethanol did not show any significant effects on this enzyme. These unusual properties suggest that this enzyme may be of significant importance for understanding the structure of the enzyme.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Academic Press Aust.|
|Keywords:||Thermoascus-Aurantiacus;Thermophilic Fungus;Purification; Endocellulase.|
|Department/Centre:||Division of Biological Sciences > Biochemistry|
|Date Deposited:||07 Dec 2010 09:43|
|Last Modified:||07 Dec 2010 09:43|
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