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Occurrence of 1-methyladenosine and absence of ribothymidine in transfer ribonucleic acid of Mycobacterium smegmatis

Vani, BR and Ramakrishnan, T and Taya, Y and Noguchi, S and Yamaizumi, Z and Nishimura, S (1979) Occurrence of 1-methyladenosine and absence of ribothymidine in transfer ribonucleic acid of Mycobacterium smegmatis. In: Journal of Bacteriology, 137 (3). pp. 1084-1087.

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Official URL: http://jb.asm.org/cgi/content/short/137/3/1084

Abstract

The minor base composition of Mycobacterium smegmatis tRNA has been studied. Thin-layer chromatographic patterns of a ribonuclease T2 digest of mycobacterial tRNA indicated the presence of appreciable amounts of 1-methyladenosine (which is commonly present only in eucaryotic tRNA), dihydrouridine, and 7-methylguanosine. Ribothymidine was absent. The S-adenosylmethionine-dependent tRNA methylases of M. smegmatis catalyzed the formation of 1-methyladenosine when Escherichia coli tRNA was used as acceptor. Similarly, E. coli extracts methylated the tRNA of M. smegmatis, forming ribothymidine.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to American Society for Microbiology.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 27 Dec 2010 06:26
Last Modified: 08 Jul 2011 07:11
URI: http://eprints.iisc.ernet.in/id/eprint/34237

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