Vani, BR and Ramakrishnan, T and Taya, Y and Noguchi, S and Yamaizumi, Z and Nishimura, S (1979) Occurrence of 1-methyladenosine and absence of ribothymidine in transfer ribonucleic acid of Mycobacterium smegmatis. In: Journal of Bacteriology, 137 (3). pp. 1084-1087.
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The minor base composition of Mycobacterium smegmatis tRNA has been studied. Thin-layer chromatographic patterns of a ribonuclease T2 digest of mycobacterial tRNA indicated the presence of appreciable amounts of 1-methyladenosine (which is commonly present only in eucaryotic tRNA), dihydrouridine, and 7-methylguanosine. Ribothymidine was absent. The S-adenosylmethionine-dependent tRNA methylases of M. smegmatis catalyzed the formation of 1-methyladenosine when Escherichia coli tRNA was used as acceptor. Similarly, E. coli extracts methylated the tRNA of M. smegmatis, forming ribothymidine.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to American Society for Microbiology.|
|Department/Centre:||Division of Biological Sciences > Microbiology & Cell Biology|
|Date Deposited:||27 Dec 2010 06:26|
|Last Modified:||08 Jul 2011 07:11|
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