Sasi, R and Nandi, US (1979) Interaction of rhodium(III) with DNA. In: Biochimica et Biophysica Acta (BBA), 563 (2). pp. 527-533.
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The nature of interaction of Rh(III) with DNA was studied using viscometry and ultraviolet, visible and infrared spectroscopy. The rate of interaction was found to be very slow at room temperature taking several days for completion. The time needed to attain equilibrium is dependent on the concentrations of metal ion, higher the concentration shorter the period required for equilibration. Visible spectra of Rh(III) were found to alter considerably in the presence of DNA. An increase in absorbance and a red shift were observed in the ultraviolet spectra of DNA in the presence of Rh(III). The specific viscosity of DNA solution was found to decrease asymptotically with time and concentrations of metal ion. The melting temperature of DNA was found to increase at lower metal ion concentrations, whereas at higher values a decrease was obtained. At still higher metal ion concentrations (Image ) a ‘nonmeltable state’ of DNA was observed. These results seem to indicate that Rh(III) binds both with the phosphate and the bases of the DNA.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to Elsevier Science.|
|Keywords:||DNA structure; Rhodium-DNA binding.|
|Department/Centre:||Division of Chemical Sciences > Inorganic & Physical Chemistry|
|Date Deposited:||23 Dec 2010 07:37|
|Last Modified:||23 Dec 2010 07:37|
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