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Cloning, expression, purification, crystallization and preliminary X-ray studies of a secreted lectin (Rv1419) from Mycobacterium tuberculosis

Patra, Dhabaleswar and Srikalaivani, R and Misra, Ashish and Singh, DD and Selvaraj, M and Vijayan, M (2010) Cloning, expression, purification, crystallization and preliminary X-ray studies of a secreted lectin (Rv1419) from Mycobacterium tuberculosis. In: Acta Crystallographica Section F, 66 (Part 1). pp. 1662-1665.

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Abstract

A secreted lectin, Rv1419, from Mycobacterium tuberculosis has been cloned, expressed, purified and crystallized and the crystals have been characterized. This represents the first X-ray investigation of a lectin or lectin-like molecule from the pathogen. The cubic crystals contain one molecule in the asymmetric unit. Sequence comparisons indicate that the lectin has a beta-trefoil fold and belongs to a well characterized family of carbohydrate-binding modules. Structural analysis of the crystals is in progress.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to John Wiley and Sons.
Keywords: bacterial lectins; Mycobacterium tuberculosis; beta-trefoil fold; sugar binding
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 03 Jan 2011 07:03
Last Modified: 03 Jan 2011 07:03
URI: http://eprints.iisc.ernet.in/id/eprint/34768

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