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Characterization and immune response of a protein produced by a cDNA clone of foot and mouth disease virus, type Asia 1 63/72

Suryanarayana, VV and Venkataramanan, R and Rao, BU and Padayatty, JD (1992) Characterization and immune response of a protein produced by a cDNA clone of foot and mouth disease virus, type Asia 1 63/72. In: Biochemistry International, 26 (6). pp. 1003-1016.

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Official URL: http://www.virushunters.net/showabstract.php?pmid=...

Abstract

A 0.9 kb double stranded cDNA of foot and mouth disease virus (FMDV) Type Asia 1, 63/72 was cloned in an expression vector, pUR222. A protein of 38 kd was produced by the clone which reacted with the antibodies raised against the virus. A 20 kd protein which may be derived from the 38 kd protein contained the antigenic epitopes of the protein VP1 of the virus. Injection of 10-20 micrograms of the partially purified 38 and 20 kd proteins or a lysate of cells containing 240 micrograms of the proteins elicited high titers of FMDV specific antibodies in guinea pigs and cattle respectively. Also, at these concentrations, the proteins protected 5 of 8 guinea pigs and 3 of 8 cattle when challenged with a virulent virus.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Academic Press Aust.
Keywords: Nucleotide-Sequence;Escherichia-Coli;Major Antigen; Polypeptides;Expression.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 25 Jan 2011 09:49
Last Modified: 25 Jan 2011 09:49
URI: http://eprints.iisc.ernet.in/id/eprint/35116

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