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Antigenic determinants of human serum retinol binding protein as probed with monoclonal antibodies

Reddy, Kumar BMMM and Karande, Anjali A and Adiga, Radhakantha P (1993) Antigenic determinants of human serum retinol binding protein as probed with monoclonal antibodies. In: Molecular Immunology, 30 (15). pp. 1355-1360.

Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/0161-5890(93)90096-T

Abstract

Monoclonal antibodies raised against human serum retinol-binding protein (hRBP) were used as probes for the study of the antigenic determinants of hRBP and those shared with the same protein from other species. The antibodies could be classified into four distinct groups and react with the homologous proteins from the rat as well as the rabbit sera. Three of these antibodies recognize sequential or continuous epitopes while the remaining antibody is directed against a discontinuous or conformational epitope. By chemical cleavage with cyanogen bromide, the domains recognized by the monoclonal antibodies could be delineated. By solid-phase synthetic approach, the core sequences recognized by two of these monoclonal antibodies were identified to amino acid sequences 45–51 and 128–131 of the primary amino acid sequence of hRBP.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Others
Date Deposited: 31 Jan 2011 07:32
Last Modified: 31 Jan 2011 07:32
URI: http://eprints.iisc.ernet.in/id/eprint/35318

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