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Characterization of poly- and monoclonal antibodies to physalis mottle (tymo) virus

Kekuda, R and Karande, AA and Savithri, HS (1993) Characterization of poly- and monoclonal antibodies to physalis mottle (tymo) virus. In: Indian Journal of Biochemistry & Biophysics, 30 (3). 151-155 .

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Official URL: http://www.niscair.res.in/ScienceCommunication/Res...

Abstract

Polyclonal antibodies were raised against the Physalis mottle virus (PhMV) and its denatured coat protein (PhMV-P). Analysis of the reactivity of the polyclonal antibodies with tryptic peptides of PhMV-P in dot-blot assays revealed that many of the epitopes were common to intact virus and denatured coat protein. Five monoclonal antibodies to the intact virus were obtained using hybridoma technology. These monoclonal antibodies reacted well with the denatured coat protein. Epitope analysis suggested that probably these monoclonal antibodies recognize overlapping epitopes. This was substantiated by epitope mapping using the CNBr digest of PhMV-P in western blots. All the five monoclonals recognized the N-terminal 15 K fragment. Attempts to further delineate the specific region recognized by the monoclonals by various enzymatic cleavages resulted in the loss of reactivity in all the cases. The results indicate that these monoclonals probably recognize epitopes within the N-terminal 15 K fragment of the coat protein.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 02 Feb 2011 07:59
Last Modified: 02 Feb 2011 07:59
URI: http://eprints.iisc.ernet.in/id/eprint/35361

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