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DNA Polymerase-β from the pupal ovaries of bombyx mori

Niranjanakumari, Somashekarappa and Gopinathan, Karumathil P (1993) DNA Polymerase-β from the pupal ovaries of bombyx mori. In: Insect Biochemistry and Molecular Biology, 23 (3). pp. 345-353.

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Official URL: http://dx.doi.org/10.1016/0965-1748(93)90018-N

Abstract

The silk glands of Bombyx mori, a highly replicative tissue contains high levels of DNA polymerases α, σ and epsilon (Porson) but not DNA polymerase-β. However, we detected the latter activity in the gonadal tissues, viz. the pupal ovaries and testes of B. mori. The enzyme has been purified to homogeneity from the pupal ovaries by a series of column chromatographic and affinity purification steps. The enzyme satisfied the criteria to be designated as DNA polymerase-β based on its small size, requirement for high concentration of monovalent cations for catalytic activity, sensitivity to ddTTP and insensitivity to aphidicolin. It is a monomeric polypeptide of Mr 40 kDa, and the Km for dNTPs ranges between 8–20 μM. DNA polymerase-β is biochemically and immunologically distinct from DNA polymerase-α from the silk glands of B. mori. The enzyme showed a preference for gapped DNA, and could not elongate ultraviolet irradiated template beyond the pyrimidine dimers. The absence of any associated primase and exonuclease activities from this enzyme, and its conspicuous absence in the highly replicative tissue, imply that it is unlikely to participate in the DNA endoreplication process.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Chromosomal endoreplication;DNA polymerases;DNA repair;DNA replication;mulberry silkworm.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Others
Date Deposited: 10 Feb 2011 07:38
Last Modified: 10 Feb 2011 07:38
URI: http://eprints.iisc.ernet.in/id/eprint/35405

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