Theoretical permutation gel electrophoretic analysis of a curved DNA fragment located in circular permutation

Nair, Murlidharan T and Madhusudan, K and Nagaraja, V and Kulkarni, BD and Majumdar, HK and Singh, Rajan (1996) Theoretical permutation gel electrophoretic analysis of a curved DNA fragment located in circular permutation. In: Electrophoresis, 17 (4). pp. 633-641.

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Abstract

Using the theoretical model for DNA curvature, we analyzed a set of fragments with a curved insert located in circular permutation. The theoretical permutation analysis of each of the cyclically located fragments reveals the presence of a shifting molecular bend locus. The delineation of the molecular bend locus associated with the fragments obtained by a second permutation helps in providing an explanation for the differential mobility behavior of the fragments.

Item Type:	Journal Article
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Keywords:	cyclically permuted;cyclically located;dna curvature; theoretical permutation gel electrophoresis
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME)
Date Deposited:	24 Aug 2005
Last Modified:	08 Feb 2012 05:26
URI:	http://eprints.iisc.ernet.in/id/eprint/3548

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