Bagga, R and Brahmachari, SK (1993) Polypurine-polypyrimidine sequences adopt unwound structure in pBR322 form V DNA as probed by single-hit analysis of HpaII sites. In: Journal of Biomolecular Structure & Dynamics, 10 (5). pp. 879-890.
Full text not available from this repository.Abstract
Structure at the polypurine-polypyrimidine sequences flanking the HpaII sites (CCGG) in pBR322 form V DNA was probed employing single-hit analysis using HpaII restriction endonuclease. Reduced cleavage efficiency of HpaII sites flanked by polypurine-polypyrimidine sequences suggested that under high torsional stress these sequences adopt unwound structures rendering these sites insensitive to restriction enzyme cleavage. In addition to polypurine-polypyrimidine sequences. HpaII sites flanked by alternating purine-pyrimidine sequence, a potential motif of left handed Z-DNA, were also found to be resistant to HpaII cleavage. Results obtained from various studies implicating structure sensitivity of restriction endonucleases and methylases were compiled and a direct correlation was observed between the occurrence of altered sites in a domain and its G/C content in pBR322 form V DNA.
| Item Type: | Journal Article |
|---|---|
| Additional Information: | Copyright of this article belongs to Adenine Press. |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
| Date Deposited: | 23 Feb 2011 05:47 |
| Last Modified: | 23 Feb 2011 05:47 |
| URI: | http://eprints.iisc.ernet.in/id/eprint/35693 |
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