Bryan, RK and Bansal, Manju and Folkhard, , W and Nave, C and Marvin , DA (1983) Maximum-entropy calculation of the electron density at 4 A resolution of Pf1 filamentous bacteriophage. In: Proceedings Of The National Academy Of Sciences of the United States of America-Biological Sciences , 80 (15). 4728-4731 .
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A 4 A electron-density map of Pf1 filamentous bacterial virus has been calculated from x-ray fiber diffraction data by using the maximum-entropy method. This method produces a map that is free of features due to noise in the data and enables incomplete isomorphous-derivative phase information to be supplemented by information about the nature of the solution. The map shows gently curved (banana-shaped) rods of density about 70 A long, oriented roughly parallel to the virion axis but slewing by about 1/6th turn while running from a radius of 28 A to one of 13 A. Within these rods, there is a helical periodicity with a pitch of 5 to 6 A. We interpret these rods to be the helical subunits of the virion. The position of strongly diffracted intensity on the x-ray fiber pattern shows that the basic helix of the virion is right handed and that neighboring nearly parallel protein helices cross one another in an unusual negative sense.
|Item Type:||Journal Article|
|Additional Information:||Copyright of this article belongs to National Academy of Sciences.|
|Department/Centre:||Division of Biological Sciences > Molecular Biophysics Unit|
|Date Deposited:||25 Feb 2011 06:03|
|Last Modified:||25 Feb 2011 06:03|
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