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Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens

Janardhan, S and Pandiaraja, P and Pandey, V and Karande, A and Kaliraj, P (2011) Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens. In: Journal of Helminthology, 85 (1). pp. 1-6.

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Abstract

The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Cambridge University Press.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 24 Mar 2011 12:03
Last Modified: 24 Mar 2011 12:03
URI: http://eprints.iisc.ernet.in/id/eprint/36191

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