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Calcium ionophore, A23187 and its amino acid complexes: spectroscopic and molecular modeling studies.

Namboodiri , K and Gaber , BP and Easwaran , ER and Balasubramanian , SV (1994) Calcium ionophore, A23187 and its amino acid complexes: spectroscopic and molecular modeling studies. In: Journal of Biomolecular Structure & Dynamics, 11 (5). 913 -926.

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Official URL: http://www.biomedsearch.com/nih/Calcium-ionophore-...

Abstract

The circular dichroism, fluorescence, Nuclear Magnetic Resonance and BLM conductance studies indicate that A23187 forms a stable complex with amino acids at low ionophore concentrations (<10(-4)M). However, A23187 prefers to be in a dimeric structure with no significant binding to amino acids, at concentrations higher than 10(-4)M. It was also observed that at lower concentrations, at which the amino acids bind to the ionophore, the affinity for calcium ions was several orders of magnitude lower than that at higher ionophore concentrations. We have also conducted molecular modeling studies to examine the structure of the A23187 dimer and its amino acid complexes. The results of these modeling studies strongly support our experimental results and validate the formation of a hydrogen bonded and energetically stable A23187 dimer and its amino acid complexes.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Adenine press.
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 25 Mar 2011 06:28
Last Modified: 25 Mar 2011 06:28
URI: http://eprints.iisc.ernet.in/id/eprint/36272

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