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Characterization of a variant glucose-6-phosphate dehydrogenase from the south Indian population.

Ramadevi , R and Savithri , HS and Bittles , AH and Rao , NA (1994) Characterization of a variant glucose-6-phosphate dehydrogenase from the south Indian population. In: Indian journal of biochemistry & biophysics, 31 (4). pp. 361-365.

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Official URL: http://www.niscair.res.in/ScienceCommunication/Res...

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) is coded by a gene on the X-chromosome. Earlier studies have shown that the South Indian population has a high incidence of this enzyme deficiency. The electrophoretic mobility, pH optimum and the K-m values for G6PD from normal and variant individuals were identical. However, the specific activity of the variant enzyme was 8 times less compared to the value of the normal enzyme. Western blot analysis of partially purified G6PD from normal and variant individuals performed using equal amounts of total protein showed that the variant protein was 3 times less in concentration. Similar analysis performed using protein corresponding to equal enzyme activity units in the normal and variant samples showed that the variant enzyme was 2.25 times less efficient compared to the normal enzyme. RNA dot blot analysis using full length G6PD cDNA probe (PGDT5B, a kind gift from Prof. L Luzzatto) revealed that lymphocytes from normal and variant individuals had equal amounts of G6PD specific mRNA.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National institute of science communication and information resources.
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 25 Mar 2011 04:59
Last Modified: 25 Mar 2011 04:59
URI: http://eprints.iisc.ernet.in/id/eprint/36278

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