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Expression of microinjected foreign DNA in silkworm,bombyx mori

Joy, O and Gopinathan, KP (1994) Expression of microinjected foreign DNA in silkworm,bombyx mori. In: Current science (Bangalore), 66 (2). 145 -150 .

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Abstract

As a prelude to achieving transgenesis in Bombyx mori, conditions have been established for successful microinjection of cloned foreign genes into the silk worm eggs. A sharpened metallic needle is used to pierce the thick chorion layer of the eggsheil, approaching through a droplet of DNA solution deposited on its surface. The microinjection is carried out within 2-2.5 h after oviposition and the injected eggs show 3-5% hatchability and 80-90% survival. Such larvae continuously expressed the microinjected cloned reporter gene, beta-galactosidase, placed under the control of a constitutively expressed cytoplasmic actin A3 gene promoter from B. mori. The expression is seen in different tissues, viz. the fat body, tracheae and the silk glands, till the late larval instars. The microinjected DNA sequences are retained in the adult G(o) moths.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Indian academy of sciences.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Others
Date Deposited: 13 Apr 2011 06:00
Last Modified: 13 Apr 2011 06:00
URI: http://eprints.iisc.ernet.in/id/eprint/36464

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